- Anti-SARS-Cov-2 Mpro ELISA Test: the first serological assay designed to detect with high specificity and sensitivity recent and past SARS-CoV-2 infection.
- This new type of serological test can detect antibodies from the onset of symptoms, allowing to confirm patients with recent and past infection.
- we are working every day to open opportunities for a better understanding of the immune response and its evolution over time after vaccination.
ANTI-SARS-COV-2 ELISA KITS
Anti-SARS-Cov-2 ELISA kit is a highly sensitive and specific test developed by Immunostep under CSIC patent licence, for the precise detection and quantitative measurement of either IgG or IgA antibodies against the Mpro (3CLpro) Protein of SARS-Cov-2 virus.
Aids in diagnosis & patient surveillance
Validated by the immunoslogy services of important hospitals and laboratories
DESIGNED TO BE USED IN HUMAN SERUM AND PLASMA SAMPLES
WHAT IS INCLUDED
Microwell plate
12×8 wells microtiter strip plate coated with recombinant SARS-CoV-2 antigen in vacuum-sealed pouch. 2 microwell sealing foils.
IgG/IgA control solutions
- 5 ml Positive Control/ Contains CMIT / MIT 3: 1 as a preservative. Ready to use.
- 5 ml cut-off. Contains CMIT / MIT 3: 1 as a preservative. Ready to use.
- 5 ml Negative Control. Contains CMIT / MIT 3: 1 as a preservative. Ready to use.
HRP-Conjugated Antihuman Antibody
120 µl of HRP-conjugated anti-human antibody (100X). Contains CMIT / MIT 3: 1 as a preservative.
Wash buffer
50 ml wash buffer (20X). HEPES, NaCl and detergents. Contains Proclin300 as a preservative (<0.0014%).
Stop Solution
12 ml Stop Solution (1X – Ready to use). 0.5M sulfuric acid (H2SO4)
Tetramethylbenzidine (TMB)
12 ml of tetramethylbenzidine (TMB) chromogenic substrate. (1X – Ready to use)
Antibody and Sample Diluent Buffer
25 ml of Antibody and Sample Diluent Buffer (1X – Ready to use). Buffer that minimizes non-specific binding, cross-reactivity and matrix interference, with blue dye. Contains CMIT / MIT 3: 1 as a preservative.
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Microwell plate
12×8 wells microtiter strip plate coated with recombinant SARS-CoV-2 antigen in vacuum-sealed pouch. 2 microwell sealing foils.
-
IgG/IgA control solutions
- 5 ml Positive Control/ Contains CMIT / MIT 3: 1 as a preservative. Ready to use.
- 5 ml cut-off. Contains CMIT / MIT 3: 1 as a preservative. Ready to use.
- 5 ml Negative Control. Contains CMIT / MIT 3: 1 as a preservative. Ready to use.
-
HRP-Conjugated Antihuman Antibody
120 µl of HRP-conjugated anti-human antibody (100X). Contains CMIT / MIT 3: 1 as a preservative.
-
Wash buffer
50 ml wash buffer (20X). HEPES, NaCl and detergents. Contains Proclin300 as a preservative (<0.0014%).
-
Stop Solution
12 ml Stop Solution (1X – Ready to use). 0.5M sulfuric acid (H2SO4)
-
Tetramethylbenzidine (TMB)
12 ml of tetramethylbenzidine (TMB) chromogenic substrate. (1X – Ready to use)
-
Antibody and Sample Diluent Buffer
25 ml of Antibody and Sample Diluent Buffer (1X – Ready to use). Buffer that minimizes non-specific binding, cross-reactivity and matrix interference, with blue dye. Contains CMIT / MIT 3: 1 as a preservative.
FAQS
You can place an order via email writing us to order@immunostep.com. Simply choose a kit and send us your order with the reference number stabished in the table. You can also contact us in +34 923 29 48 27 (Spain).
CE/IVD kits are only suitable for clinical use in European Union and countries with EU conformity recognition agreements. Manufactured in Spain by Immunostep under CSIC Patent Licence.
These tests are for professional use only. If you are interested in acquiring these kits, please, contact us by email info@immunostep.com, and we will contact you shortly.
If you are a particular and you want to acquire this test, please contact us in order to recieve a personalized proposal of distributors near you.
Our products are available through our extensive distribution network all over the world. Please contact us and we will give you all the information shortly.
We strive to maintain sufficient inventory to meet your needs. In the rare case that a product is out of stock, you will be notified at the time of your order.
Protocol:
- Add sample Incubation Add 100 µl of the positive control, the negative control, the calibrator and the prepared sample (100:1 dilution) into the individual wells of the microplate. It is recommended to use two wells per sample, including the controls and in particular the calibrator. Incubate for 60 minutes at +37°C. When the process is manual, cover the microplate with one of the protective foils provided.
- Washing If necessary, remove the protective film. Empty the wells and then wash 4 times using 300 µl of 1X wash buffer in each wash. Leave the wash buffer in each well for 30 to 60 seconds per wash cycle. After washing, completely remove all liquid from the microplate by tapping it on absorbent paper with the openings facing downwards to remove all residual wash buffer.
- Incubation of the conjugate Add 100 µl of the dilution (1:100) of the HRP-conjugated antibody to the wells and incubate for 30 minutes at +37°C. When the process is manual, cover the microplate with one of the protective films provided.
- Washing If necessary, remove the protective film. Empty the wells and wash as described above (step 2).
- Substrate incubation Add 100 µl of the chromogenic substrate solution (TMB) to each well of the microplate. Incubate for 10 minutes at room temperature (+18°C and +24°C) and protected from light.
- Stopping Add 100 µl of the stop solution (1X – ready to use) to each well, trying to follow the same order in which the substrate solution was added.
- Absorbance measurement Measure the optical densities (OD) of each well in a microplate spectrophotometer at 450 nm and a reference wavelength of between 620 and 650 nm within 30 minutes of adding the stopping solution.
- Before measurement, carefully shake the plate to ensure an even distribution of the solution.
