- Anti-SARS-Cov-2 Spike ELISA designed to monitor with high specificity and sensitivity SARS-CoV-2 immune response.
- This serological assay can detect antibodies generated as a response to SARS-CoV-2 natural infection and vaccination in the same assay.
- We are working every day to open opportunities for a better understanding of the immune response and its evolution over time.
SARS-COV-2 ELISA Spike Kit
Anti-SARS-Cov-2 Spike ELISA kit is a highly sensitive and specific test developed by Immunostep for the precise detection of either IgG antibodies against the Spike Protein of the SARS-Cov-2 virus. This kit is able to identify antibodies produced as a response to natural infection, and as a response to vaccination.
AIDS IN DIAGNOSIS & PATIENT SURVEILLANCE
HIGH RELIABILITY IDENTIFYING ANTIBODIES AS A RESULT OF VACCINATION AND NATURAL INFECTION.
DESIGNED TO BE USED IN HUMAN SERUM AND PLASMA SAMPLES
What is Included
MICROWELL PLATE
12×8 wells microtiter strip plate coated with recombinant SARS-CoV-2 antigen (S Protein) in vacuum-sealed pouch. 2 microwell sealing foils.
CONTROL SOLUTIONS IGG | IGA
- 5 ml Positive Control/ Contains CMIT / MIT 3: 1 as a preservative. Ready to use.
- 5 ml Negative Control. Contains CMIT / MIT 3: 1 as a preservative. Ready to use.
CUT-OFF IGG | IGA
5 ml cut-off. Contains CMIT / MIT 3: 1 as a preservative. Ready to use.
HRP-CONJUGATED ANTIHUMAN ANTIBODY
120 µl of HRP-conjugated anti-human antibody (100X). Contains CMIT / MIT 3: 1 as a preservative.
WASH BUFFER
50 ml wash buffer (20X). HEPES, NaCl and detergents. Contains Proclin300 as a preservative (<0.0014%).
STOP SOLUTION
12 ml Stop Solution (1X – Ready to use). 0.5M sulfuric acid (H2SO4)
TMB
12 ml of tetramethylbenzidine (TMB) chromogenic substrate. (1X – Ready to use)
DILUENT BUFFER
25 ml of Antibody and Sample Diluent Buffer (1X – Ready to use). Buffer that minimizes non-specific binding, cross-reactivity and matrix interference, with blue dye. Contains CMIT / MIT 3: 1 as a preservative.
-
MICROWELL PLATE
12×8 wells microtiter strip plate coated with recombinant SARS-CoV-2 antigen (S Protein) in vacuum-sealed pouch. 2 microwell sealing foils.
-
CONTROL SOLUTIONS IGG | IGA
- 5 ml Positive Control/ Contains CMIT / MIT 3: 1 as a preservative. Ready to use.
- 5 ml Negative Control. Contains CMIT / MIT 3: 1 as a preservative. Ready to use.
-
CUT-OFF IGG | IGA
5 ml cut-off. Contains CMIT / MIT 3: 1 as a preservative. Ready to use.
-
HRP-CONJUGATED ANTIHUMAN ANTIBODY
120 µl of HRP-conjugated anti-human antibody (100X). Contains CMIT / MIT 3: 1 as a preservative.
-
WASH BUFFER
50 ml wash buffer (20X). HEPES, NaCl and detergents. Contains Proclin300 as a preservative (<0.0014%).
-
STOP SOLUTION
12 ml Stop Solution (1X – Ready to use). 0.5M sulfuric acid (H2SO4)
-
TMB
12 ml of tetramethylbenzidine (TMB) chromogenic substrate. (1X – Ready to use)
-
DILUENT BUFFER
25 ml of Antibody and Sample Diluent Buffer (1X – Ready to use). Buffer that minimizes non-specific binding, cross-reactivity and matrix interference, with blue dye. Contains CMIT / MIT 3: 1 as a preservative.
Qualitative and Quantitative Assay
Immunogenicity results are reported as an international standard unit (BAU/mL)
FAQS
You can place an order via email writing us to order@immunostep.com. Simply choose a kit and send us your order with the reference number stabished in the table. You can also contact us in +34 923 29 48 27 (Spain).
CE/IVD kits are only suitable for clinical use in European Union and countries with EU conformity recognition agreements. Manufactured in Spain by Immunostep under CSIC Patent Licence.
This ELISA kit against the S Protein has been calibrated against the WHO First International Standard for anti-SARS-CoV-2 immunoglobulin (human), NIBSC Code 20/136. In this way, it is possible to quantitatively report the concentration of IgG immunoglobulins in United Antibody Units (BAU / ml) against protein S. For this, the kit includes a positive control of known concentration with which to make a calibration line through serial dilutions.
These tests are for professional use only. If you are interested in acquiring these kits, please, contact us by email info@immunostep.com, and we will contact you shortly.
If you are a particular and you want to acquire this test, please contact us on info@immunostep.com in order to recieve a personalized proposal of distributors near you.
Our products are available through our extensive distribution network all over the world. Please contact us and we will give you all the information shortly.
We strive to maintain sufficient inventory to meet your needs. In the rare case that a product is out of stock, you will be notified at the time of your order.
